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The chloroplast genome of peanut was used as a model;55 coding sequences were retrieved,and codon usage of these genes was analyzed using bioinformatics tools.The results showed that: The GC content(including that of the entire sequence a_/n d at the first(GC1),second(GC2),and third(GC3) codon positions)of peanut chloroplast coding genes was less than 50%,indicating an AT bias in base composition;among 29 synonymous cod_/ons with relative synonymous codon usage(RSCU) values > 1,only one terminates with G;thus,codon usage exhibits a strong bias toward AT at the third codon position;neutrality plot and Nc-plot analyses revealed that natural selection was the dominant force shaping codon usage bias in the peanut chloroplast genome;thirteen optimal codons including TTA,TTG,and ATT were identified based on high RSCU values.These findings enhance our understanding of codon usage patterns in the peanut chloroplast genome,facilitate chloroplast transgene expression optimization,and provide a foundation for peanut genetic improvement via chloroplast engineering.
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Basic Information:
China Classification Code:S565.2
Citation Information:
[1]ZHANG Bo,YU Ming-hui,LI Hang-yu.Analysis of Codon Usage Bias in the Peanut Chloroplast Genome[J].Journal of Anhui Agricultural Sciences().
2026-10-19
2026-10-19
2026-10-19